Real Science vs Bro Science: Ohhh some of you are NOT going to like this!

[derek420colorado]

So it would seem taking taking a 6in clone or so after a plant u like flowers and then do ur culture so it's got size to its advantage and then continue ?

Not really, the whole name of the game is sterile sterile sterile and the less tissue you have the less surface area to get contaminated

 

[Fitzy]

If it works, it would certainly give the lil lady a head start! I like the thinking.

It makes sense the only problem I see is mostly it would be a good breeding tool but it takes awhile from wat I was reading so not sure how commercial it would be as far as flower production but it's absolutely awsome and I definitely hope to try this when I get moved eventually with more room definitely worth more investigation

 

[Fitzy]

Not really, the whole name of the game is sterile sterile sterile and the less tissue you have the less surface area to get contaminated

Then may only be suitable for photos as the pics and time frames they were discussing made it seem long and that there are way to sterilize the tissue am I miss interpreting? Either way it's very cool and warrants further investigating

 

[derek420colorado]

Then may only be suitable for photos as the pics and time frames they were discussing made it seem long and that there are way to sterilize the tissue am I miss interpreting? Either way it's very cool and warrants further investigating

yep you clean the exoplants with sterilized water. but it’s about when you do your transfers. IE from jar of sterile water to the agar dish. Whenever it is in contact with air it is at high likelihood of contamination. All you have to do is get an agar dish that is sterile and open it and wave it around in the air. In a week it will be growin 3 or 4 different types of mold on fungus and stuff. So when you do the transfers it’s best to have smaller tissue size. At least this is the case in mycology and I think the same principle would apply

 

[derek420colorado]

Also if it takes too long for a commercial application it can definitely be used for breeding.

 

[bil]

yes, breeding operations is the golden ticket.

Found a winning pheno of an auto? Well now you CAN clone it!

 

[pop22]

The explants don't root until rooting hormone is introduced. I suspect, though obviously don't know, that they can stay as explants as long as any other. My understanding is companies using this technique can store tens of thousands of explants for months. If this part works the same for autos, we have a winner!

here is what nebula said:

I have been talking to a company , (name removed) "which has been experimenting on tissue culture with cannabis plants, including autos. They said once an auto-flowering plant grown in tissue culture has made roots, it's like the clock has been reset and the plant starts growing and flowering on its own like an auto that's been grown from seed. They told me they don't know of a way to maintain the vegetative stage after that. The only thing you can do is start another culture once the buds have formed."

 

[Fitzy]

The explants don't root until rooting hormone is introduced. I suspect, though obviously don't know, that they can stay as explants as long as any other. My understanding is companies using this technique can store tens of thousands of explants for months. If this part works the same for autos, we have a winner!

That's exactly wat I'm wondering I hope it will be a viable solution

 

[Son of Hobbes]

The explants don't root until rooting hormone is introduced. I suspect, though obviously don't know, that they can stay as explants as long as any other. My understanding is companies using this technique can store tens of thousands of explants for months. If this part works the same for autos, we have a winner!

How long does it take an explant to propagate into a seedling though?

 

[pop22]

Don't remember for sure but I think its 10-21 days

How long does it take an explant to propagate into a seedling though?